A bioinformatics approach to design a novel epitope-based vaccine against Simian Immunodeficiency Virus (Retroviridae: Lentivirus)
Abstract
Simian Immunodeficiency Viruses (SIV) have been found to naturally infect African nonhuman primates (NHP). This causative agents are important and one of the special interest as the root cause of the HIV/AIDS pandemic, one of the most threatening infectious diseases worldwide. The aim of this study was to design an epitope-based vaccine using bioinformatics approaches of the circulating SIV in Kenya, Africa. In this study, we used 17 partial SIV envelope glycoprotein genes retrieved from GenBank® (National Center for Biotechnology Information, USA). We analysed the candidate epitopes using the Immune Epitope Database and Analysis Resource. Then, we performed the protective antigens prediction usingVaxiJen. Interestingly, this study revealed the data of B cell epitope prediction, protective antigens prediction, and molecular phylogenetic of circulating SIV in Kenya, Africa. In sum, this study can be used to design a novel epitope-based vaccine against SIV. We suggest further studies to conduct confirmatory experiments (in vitro and in vivo).
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References
Hayward A. Origin of the retroviruses: when, where, and how?. CurrOpinVirol, 2017; 25: 23-27.
Bailes E, Gao F, Bibollet-Ruche F, Courgnaud V, Peeters M, Marx PA, Hahn BH, Sharp PM. Hybrid origin of SIV in chimpanzees. Science, 2003; 300: 1713.
Greenwood EJD, Schmidt F, Kondova I, Niphuis H, Hodara VL, Clissold L, McLay K, Guerra B, Redrobe S, Giavedoni LD, Lanford RE, Murthy KK, Rouet F, Heeney JL. Simian immunodeficiencyvirusinfection of chimpanzees (Pan troglodytes) shares features of bothpathogenic and non-pathogenic lentiviralinfections. PLoS Pathog, 2015; 11(9): e1005146.
Takemura T, Ekwalanga M, Bikandou B, Ido E, Yamaguchi-Kabata Y, Ohkura S, Harada H, Takehisa J, Ichimura H, Parra HJ, Nende M, Mubwo E, SepoleM, Hayami M, Miura T. A novel simian immunodeficiency virus from black mangabey (Lophocebusaterrimus) in the Democratic Republic of Congo. J Gen Virol, 2005; 86: 1967-1971.
Yang C, Spies CP, Compans RW. The human and simian immunodeficiency virus envelope glycoprotein transmembrane subunits are palmitoylated. Proc Natl Acad Sci USA, 1995; 92(21): 9871-9875.
OyarzúnP,KobeB. Recombinant and epitope-based vaccines on the road to the market and implications for vaccine design and production. Hum VaccinImmunother, 2016; 12(3): 763-767.
Adianingsih OR, Kharisma VD. Study of B cell epitopeconservedregion of the Zika virusenvelopeglycoprotein to developmulti-strain vaccine. J Appl Pharm Sci, 2019; 9(1): 98-103.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol, 2013; 30(12): 2725-2729.
Doytchinova IA, Flower DR. Vaxijen: aserver for prediction of protectiveantigens, tumourantigens and subunitvaccines. BMC Bioinformatics, 2007; 8(4): 1-7.
Hilyatuz Z, Ma'rup A, Tambunan USF, Parikesit AA. Immunoinformaticsapproach in designing epitope-based vaccine against meningitis-inducing bacteria (Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae Type B). Drug Target Insights, 2016; 10: 19-29.
Fosgerau K, Hoffmann T. Peptide therapeutics: current status and future directions. Drug Discov Today, 2015; 20: 122-128.
Lei Y, Zhao F, Shao J, Li Y, Li S, Chang H, Zhang Y. Application of built-in adjuvants for epitope-based vaccines. PeerJ, 2019; 6: e6185.
Hajissa K, Zakaria R, Suppian R, Mohamed Z.Epitope-based vaccine as a universal vaccination strategy against Toxoplasma gondii infection: a mini-review. J Adv Vet Anim Res, 2019; 6(2): 174-182.
Choi KS, Lee EK, Jeon WJ, Kwon JH. Antigenic and immunogenicinvestigationof the virulencemotif of the Newcastle diseasevirusfusionprotein. J Vet Sci, 2010; 11(3): 205-211.
Sanchez-Trincando JL, Gomez-Perosanz M, Reche PA. Fundamentals and methods for T-and B-cell epitope prediction. J Immunol Res, 2017; 2017: 2680160.
Gorbalenya AE, Lauber C. 2017. Phylogeny of Viruses. In: Reference Module in Biomedical Sciences, Elsevier.
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